gene targeting การใช้

• Eight weeks after transfection, the plants can be screened for gene targeting via PCR.
• He wrote the first book on gene targeting in 1992.
• By homologous recombination mediated gene targeting those mice became deficient in mGlu receptor 1 protein.
• They also allow precise genetic modifications by gene targeting.
• Also called gene targeting, the method is especially common in Nobel Prize for Physiology or Medicine.
• Homologous recombination is also used in gene targeting, a technique for introducing genetic changes into target organisms.
• He developed the first knock-in gene, now a widely employed approach in gene targeting and gene editing.
• The technique of gene targeting uses homologous recombination to target desired changes to a specific endogenous gene.
• "' Gene targeting "'( also, replacement strategy based on homologous recombination ) is a vector for each gene of interest.
• He was standing in a pitch-black darkroom, developing X-ray film that would reveal whether he had succeeded in gene targeting.
• The frequency of gene targeting can be greatly enhanced with the use of engineered nucleases such as zinc finger nucleases,
• Furthermore, certain technologies enable the knock-in of a particular mutation rather than just knock-outs associated with older gene targeting technologies.
• Gene targeting through ZFNs or TALEN-based approaches can also be used to modify defective genes at their endogenous chromosomal locations.
• Marth and Rajewsky reported in 1994 that Cre-lox recombination could be used for conditional gene targeting in developing T-cells in mice.
• In turn, the other TLR genes were ablated in mice by gene targeting, largely in the laboratory of Shizuo Akira and colleagues.
• Loops with CTCF and cohesin at the start and end of the loop that restrict enhancer-gene targeting are  insulated neighborhoods . 
• While at the University of Wisconsin in the 1980s, Smithies developed gene targeting in mice, a method of replacing single mouse genes using homologous recombination.
• He plunged into molecular biology and molecular genetics when the fields began in the 1970s, leading to his discovery, in the 1980s, of gene targeting.
• Mario R . Capecchi, Ph . D . won the 2007 Nobel Prize in Physiology or Medicine as a University of Utah gene targeting pioneer.
• As mosses are haploid organisms, the regenerating moss filaments ( protonemata ) can be directly assayed for gene targeting within 6 weeks using PCR methods.